Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 1KG
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 1KG
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Growcells PBS 1X 1L EA
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Growcells TAE BUFFER 50X 2L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-100.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 100 Grams
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Research Products International Corp NP-40 Lysis High Salt Buffer Solution, 500 ML
1X buffer used for extracting proteins from cells.
![Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B75000-100.0.JPG-250.jpg)
Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams
Substance Name: bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Formula: C8H19NO5
Molecular Weight:?209
Chemical Purity:?>99%
Soluble in: Water
Store at:?Room Temperature
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-100.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-500.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 500 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
Research Products International Corp Bicine Ultra-Pure, 100 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Research Products International Corp PBS, 10X Powder Concentrate, 10 L
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
Biotium Blocking Buffers Background Reducers Fluorescence Microscopy Chemical Reagents TrueBlack 1/EA
This buffer is a component of the TrueBlack Background Suppressor System (Permeabilizing) It is an excellent general use non-mammalian blocking buffer with detergent for intracellular immunofluorescence Not recommended for use with HRP (contains azide) 100 mL bottle
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Research Products International Corp MES, Anhydrous, 250 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
![Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92060-25.0.JPG-250.jpg)
Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams
- Substance Name: 3-(N-Morpholino)2-hydroxypropanesulfonic acid
- CAS Number: 68399-77-9
- Molecular Formula: C7H15NO5S
- Molecular Weight: 225.3