Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TT - SDS Running Buffer Kit (10X), 1 L
TT-SDS Running Buffer 10X KitEach kit contains both a cathode and anode buffer (Catalog #: 765540 and 765542)
Bioworld TBE Buffer w/ Sodium for Resolve Gels (1X), 40 PK(s)
Product DescriptionTBE (Tris-Borate-EDTA) buffer is for resolve gels that comes with sodium and has a concentration of 1X. Each pack has 500 mL of pre-weighed powder mix, product comes with 20 packs.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
![Alkali Scientific bis TRIS [bis (2 hydroxyethyl)aminotris(hydroxymethyl)methane], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659894.jpg-250.jpg)
Alkali Scientific bis TRIS [bis (2 hydroxyethyl)aminotris(hydroxymethyl)methane], 500 G
Bis TRIS is a high-purity biological buffer available in a 500g powder form. It is used in various biochemical and molecular biology applications where a stable pH above 7.0 is required. Bis TRIS is an ideal buffer for enzyme assays, protein electrophoresis, and DNA/RNA analysis due to its strong buffering capacity in alkaline conditions. The 500g size offers a convenient amount for medium-scale research operations or routine laboratory use. Its minimal ionic interference and consistent pH control make it suitable for experiments that require high precision, especially in applications related to protein and nucleic acid studies. Bis TRIS is a commonly used reagent in the life sciences and pharmaceutical industries.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 20L-CBY
Tris-HCl Buffer (1 M pH 7 4) - 20L-CBY (For Research Use and Further Manufacturing Only)
Crescent Chemical Co Inc TE BUFFER (100X)
TE Buffer (100X) 100 x concentrated aqueous solution. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42554.01
Bioworld Tris/Glycine Buffer for Resolve Gels (1X), 20 PK(s)
1X Tris/Glycine Buffer for Resolve GelsBuffer System: Pre-weighed powder mix in pouches (packs)1 pouch(pack)= 500 mL
Medchemexpress LLC NVP-TAE 684 | 761439-42-3 | 99.7% | 614.20 g/mol | C30H40ClN7O3S | 100 MG
NVP-TAE 684 is a potent, selective anaplastic lymphoma kinase (ALK) inhibitor used in research to block proliferation of ALCL-derived and ALK-dependent cell lines. It shows low-nanomolar activity and is supplied as a high-purity small molecule for in vitro studies and compound screening.
- Potent ALK inhibition with IC50 in the low-nanomolar range (≈2-10 nM).
- Selective activity against ALK-dependent and ALCL-derived cell lines.
- Suitable for in vitro research and compound screening applications.
- Available as solid and DMSO solution formats and multiple pack sizes.
- High purity suitable for research use (>99.7%).
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005313 TRIS HYDROCHLORIDE 100G
Bioworld TE (Tris-EDTA) Buffer, Sterile, 500 mL
Product DescriptionTE (Tris-EDTA) is a sterile buffer that has a pH level of 8.0 brought up with HCl and is stored at room temperature. It contains 10 mM Tris and 1 mM EDTA. It is of biotechnology grade and no DNAse activity is detected.TE buffer is used to solubilize DNA or RNA while protecting it from degradation. Commonly used for DNA isolation and electrophoresis protocols.
Research Products International Corp HEPES, Sodium Salt, 5 KG
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
![Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T32020-10000.0.JPG-250.jpg)
Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Bioworld TBS Buffer, Low Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Biotium Ready-to-Use Tyramide Amplification Buffer, 1X, 25ML
Ready-to-use tyramide amplification buffer can be used for tyramide signal amplification with CF Dye Tyramides or other tyramides without the need to add hydrogen peroxide.
Bioworld MOPS-SDS Running Buffer (20X), 500 mL
MOPS-SDS Running Buffer for Protein Gel electrophoresisSDS running buffer with MOPS and Tris (pH 7.7)20X concentrationAll bioWORLD running buffers are prepared with high quality reagentsSpecifications:Ready to use, just dilute in distilled water. Save time and ensure reproducible results.1X contains: 50mM MOPS, 50mM Tris base, 1mM EDTA, and 0.1% SDS
Bioworld TBE Urea Running Buffer (10X), 4 L
Product DescriptionTBE(Tris-Borate-EDTA) urea running buffer is used for protein analysis. It has a 10X concentration and contains 0.89M Tris base, 0.89M boric acid, 20 mM EDTA, and a pH at 8.3.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.